Gene expression profile at single cell level of fibroblasts from untreated (UT) and imiquimod (IMQ)-induced mouse skin.
Summary:
We used single-cell RNA sequencing (scRNA-seq) to analyze the heterogeneity of dermal fibroblasts in homeostasis and skin inflammation.
Overall Design:
Dermal fibroblasts of the UT/IMQ-induced PdgfraDreER-tdTomato mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of tdTomato signal and analyzed using scRNA-seq.
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