Single Cell RNA Sequencing of neonatal and juvenile mice skin (wound and unwounded)
Summary:
We perform 10X Genomics scRNA-Seq on P2, P21, P2 + 7 days post wound, P21 + 7 days post wound, with 3 bioreplicates for each condition. Fastq files and output folders were generated from Cellranger pipeline. Loom files were generated using Velocyto.py for downstream analysis. Chemistry: V2
Overall Design:
scRNA-Seq on P2, P21, P2 7 days post wound, P21 7 days post wound, with 3 bioreplicates for each condition
Cell Clustering
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Enrichment Analysis
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Top pathways
Ranked by absolute NES
Differentially Expressed Genes
p-value threshold0.05
Maximum adjusted p-value
Log fold change1.0
Minimum log₂ fold change
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logFC
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CellPhoneDB Cell-Cell Communication Analysis
Dynamic Analysis
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