mice: C57BL/6 mouse model: IMQ-induced psoriasis model treatment: Treated with 62.5 mg IMQ
Experiment Information
Title:
Single-cell transcriptomics of the mouse skin reveal potential target of psoriasis
Summary:
Psoriasis is a complex chronic inflammatory skin disease, but the exact mechanism underlying its development remains elusive. We found the expression of SHP2 was significantly higher in skin lesions and human peripheral blood mononuclear cells derived from psoriatic patients than those from normal controls. determine whether SHP2 activity affects psoriasis pathogenesis, we treated the murine psoriatic model with SHP099, a potent allosteric inhibitor of SHP2. SHP099 ameliorates the IMQ-induced psoriatic development in mice with unclear molecular mechanism. To address that, single-cell RNA sequencing was performed to explore the role of SHP2 in all cell types in IMQ-induced mouse skin upon SHP099 treatment.
Overall Design:
Examination of 6 skin samples in IMQ-induced psoriasis-like mouse model
Cell Clustering
Color UMAP by
Switch between fine-grained and coarse annotations
Loading UMAP…
Enrichment Analysis
Enrichment mode
Select enrichment collection
Top pathways
Ranked by absolute NES
Differentially Expressed Genes
p-value threshold0.05
Maximum adjusted p-value
Log fold change1.0
Minimum log₂ fold change
Cell type group
Filter by cell type
Gene
logFC
p-value
Score
Group
CellPhoneDB Cell-Cell Communication Analysis
Dynamic Analysis
Select Cell Types
Choose 2 or more cell types to analyze ligand-receptor interactions
